The Green Spine is a multi-use path across the middle of Tampa. I rode it east from Howard Ave to 17th Ave, 3.2 miles, and the whole ride stays on the trail. The path is fully separated from cars the entire way.
It crosses North Blvd and runs right through downtown at Ashley Dr, Tampa St and Florida Ave, then keeps going past Nebraska Ave, Palm Ave and Avenida Republica de Cuba before finishing at 17th Ave.
The video is real time except for the red-light waits, which I sped up.